目的 建立反相高效液相色谱法(RP-HPLC)同时测定飞龙掌血药材中橙皮苷、氯化两面针碱, 白屈菜红碱, 毛两面针素的含量。方法 采用优化色谱条件;色谱柱:Diamonsil-C₁₈柱(4.6 mm×250 mm, 5 μm);流动相:乙腈(A)-0.75%冰醋酸(B);梯度洗脱;检测波长:290 nm;流速:1.0 mL·min^-1;紫外检测器检测;分别测出其峰面积, 采用外标法定量;柱温:30 ℃。结果 飞龙掌血中橙皮苷、氯化两面针碱、白屈菜红碱、毛两面针素的线性范围分别为0.487 4~2.437 2 μg(r=0.999 7), 0.030 30~0.151 5 μg(r=0.999 2), 0.062 34~0.311 7 μg(r=0.999 3), 0.024 92~0.124 6 μg(r=0.999 9);平均加样回收率(n=6)分别为100.76%(RSD 2.75%)、97.98%(RSD 1.25%)、100.07%(RSD 3.24%)、100.10%(RSD 3.83%)。结论 该方法简便、快速、准确, 重现性好, 回收率高, 可用于飞龙掌血药材中4种主要化学成分含量的测定, 其结果可为其质量控制、合理用药及进一步研究开发提供参考。

OBJECTIVE To develop a RP-HPLC method for simultaneous determination of hesperidin, nitidine chloride, chelerythrine, and toddalolactone in Toddalia asiatica(L.)Lam.METHODS The RP-HPLC system consisted of a Diamonsil C₁₈ column (4.6 mm×250 mm, 5 μm) with the mobile phase of acetonitrile-water solution containing 75% glacial acetic acid. UV detector was used, and the detection wave length was 290 nm. Gradient elution was adopted at a flow rate of 1.0 mL·min^-1, and the column temperature was 30 ℃. For different constituents, external standard method was used with the peak area at 290 nm as the quantitative index. RESULTS The liner ranges of hesperidin, nitidine chloride, chelerythrine and toddalolactone were 0.487 4-2.437 2 μg(r=0.999 7), 0.030 3-0.151 5 μg(r=0.999 2), 0.062 3-0.311 7 μg(r=0.999 3), and 0.024 9-0.124 6 μg(r=0.999 9), respectively. The average recoveries (n=6) were 100.76%(RSD 2.75%), 97.98%(RSD 1.25%), 100.07%(RSD 3.24%), and 100.10%(RSD 3.83%), respectively. CONCLUSION The method is accurate, simple, rapid, and reproducible for the determination of hesperidin, nitidine chloride, chelerythrine, and toddalolactone in Toddalia asiatica(L.)Lam. The determination result can be used as a reference for the rational medication, quality control, and further study of Toddalia asiatica(L.)Lam.